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stemdifftm trilineage differentiation kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc stemdifftm trilineage differentiation kit
    Stemdifftm Trilineage Differentiation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemdifftm+trilineage+differentiation+kit/stemdifftm+trilineage+differentiation+kit/pmc11384962__mmc2-1018-39-44
    Average 90 stars, based on 1 article reviews
    stemdifftm trilineage differentiation kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: CHD7 regulates definitive endodermal and mesodermal development from human embryonic stem cells
    Article Snippet: hESCs were induced to generate DE by the PSC Definitive Endoderm Induction Kit (Thermo Fisher Scientific, Rockford, IL) according to the manufacturer’s instructions. hESCs were induced to differentiate into ectoderm or mesoderm by the STEMdiffTM Trilineage Differentiation Kit (STEMCELL Technologies).

    Article Title: Generation of two pairs of induced pluripotent stem cells from Angelman syndrome patients with class I 15q11.2-q13.1 deletions and their unaffected familial controls.
    Article Snippet: Trilineage differentiation potential was assessed using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies, 05230) following manufacturer’s instructions.

    Software:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: .. Scale bar, 1 mm. (B) Exemplification of the image processing steps done by the Harmony v4.0 software script to detect the SiR-DNA labeled hiPSCs from all imaged wells in each well (1), filter the images by applying a Sliding Parabola algorithm (2) or a Mean Smoothing algorithm (3) and then identify the area occupied by the whole Image Region (4). qPCR quantification of expression markers of ectodermal SOX1 (C) and PAX6 (D), endodermal SOX17 (E) and LEFTY1 (F) and mesodermal NCAM (G), TBXT (H) and CXCR4 (I) markers from BJ WT hiPSCs cultured in STEMdiffTM Trilineage Differentiation Kit (STEMCELL). ..

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-IgG Santa Cruz sc2025; RRID: AB_737182 Mouse anti-SMN Novus Biologicals NB100-1936; RRID: AB_531374 Rabbit anti-Gemin2 Abcam ab150383; RRID: AB_3099716 Rat anti-SOX2 Invitrogen 14-9811-82; RRID: AB_11219471 Mouse anti-NESTIN Millipore MAB353; RRID: AB_94911 Rabbit anti-ISL1 Abcam ab109517; RRID: AB_10866454 Mouse anti-NKX6.1 Developmental Studies Hybridoma Bank F55A10; RRID: AB_532378 Mouse anti-MAP2 Novus Biologicals NBP2-25156; RRID: AB_3099715 Rabbit anti-TBXT Cell Signaling 81694; RRID: AB_2799983 Mouse anti-ACTA2 Sigma-Aldrich A2547; RRID: AB_476701 Rabbit anti-CHAT Proteintech 20747-1-AP; RRID: AB_10898169 Mouse anti-SMI32/anti-Neurofilament H (NF-H) Biolegend 801701; RRID: AB_2564642 Rabbit anti-Desmin Abcam ab15200; RRID: AB_301744 Mouse anti-Titin Novus Biologicals NB600-1206; RRID: AB_10003288 Mouse anti-b-Actin Cell Signaling 8H10D10; RRID: AB_2242334 Rabbit anti-b-Tubulin Cell Signaling 2146; RRID: AB_2210545 Chemicals, peptides, and recombinant proteins mTESR1 STEMCELL Technologies 85850 ReLeSR STEMCELL Technologies 100–0484 Advanced DMEM/F12 Thermo Fisher Scientific 12634028 Neurobasal Medium Thermo Fisher Scientific 21103049 GlutaMAX Thermo Fisher Scientific 35050087 2-Mercaptoehtanol Life Technologies 21985023 GlutaMAX Thermo Fisher Scientific 35050087 Ascorbic Acid SigmaAldrich A4403 B27 Thermo Fisher Scientific 17504044 N2 Thermo Fisher Scientific 17502048 Penicillin-Streptomycin Life Technologies 15140122 Matrigel Corning 354234 TRIzol Life Technologies 15596026 Laminin Thermo Fisher Scientific 23017015 Poly-D-lysine Sigma-Aldrich A-003-E TissueTek OCT Sakura Finetek 4583 Sucrose Millipore 107651 MLN-4924 Active Biochem MLN-4924 Cycloheximide Sigma-Aldrich C1988 Y-27632 (ROCKi) Hölzel A11001-50 FGF-2 Millipore 32160702 SB431542 Bio-Techne 1614/10 LDN193189 Hölzel M1873) RA Sigma-Aldrich R2625 SAG Millipore 566660 Ara-C Sigma-Aldrich C1768 (Continued on next page) Cell Reports Medicine 5, 101659, August 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAPT Tocris 2634/10 BDNF Qkine QK050 GDNF Qkine QK051 Papain Worthington LK003178 DNase Worthington LK003172 Accutase Corning 25-058-Cl Vectashield Antifade Mounting Medium Biozol Diagnostica VEC-H-1000 SiR-DNA Spyrochrome SC007 Hoechst33342 Life Technologies H3570 Critical commercial assays STEMdiffTM Trilineage Differentiation Kit STEMCELL Technologies 05230 Deposited data scRNA-seq This paper EGAS00001007259 Experimental models: Cell lines BJ siPS-D Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi005-A, Table S2 1016A Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi007-A, Table S2 38D-I Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi015-A, Table S2 51N-II Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi017-A, Table S2 39C-III Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi016-A, Table S2 CRTD1 CRTD Stem Cell Core Facility (Volkner et al.81) CRTDi004-A, Table S2 BJ-SMN:Clover This paper N/A 38D-I isogenic corrected clone #2 This paper N/A 38D-I Isogenic corrected clone #4 This paper N/A 51N-II Isogenic corrected clone #1 This paper N/A 51N-II Isogenic corrected clone 2 This paper N/A 39C-III Isogenic corrected clone 9 This paper N/A 39C-III Isogenic corrected clone 10 This paper N/A Experimental models: Organisms/strains Mouse: Smn+/ ;hSMN2+/+; hSMND7+/+; Hb9:GFP+ Rodriguez-Muela et al.18,37 N/A Oligonucleotides Single-stranded DNA oligos for cloning IDT See Table S3 Primers for PCR and Sanger Sequencing IDT See Table S3 Primer for RT-qPCR IDT See Table S3 Recombinant DNA pTG-Cr-SMN This paper N/A pTG-HR-SMN:Clover This paper N/A Software and algorithms GraphPad Prism 10.0.3 Graphpad Software, Inc. https://www.graphpad.com/features Geneious Prime 2023.0.3 Biomatters Ltd https://www.geneious.com/ ImageJ software NIH https://imagej.net/ij/ Arivis Vision4D 3.3.0 Zeiss https://www.arivis.com/ Arivis SIS Zeiss https://www.arivis.com/products/ sis-converter (Continued on next page) e2 Cell Reports Medicine 5, 101659, August 20, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Columbus Analysis System PerkinElmer https://www.perkinelmer.com/de/lab- products-and-services/product-support. html#Columbus CellRanger software (v7.1.0) 10X Genomics https://www.10xgenomics.com/support Seurat pipeline (v. 4.3.0) Satija Lab https://satijalab.org/seurat/ R (v. 4.2.2, with Bioconductor 3.16) The R Foundation https://www.r-project.org ZEN Zeiss https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html Article ll OPEN ACCESS

    Labeling:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: .. Scale bar, 1 mm. (B) Exemplification of the image processing steps done by the Harmony v4.0 software script to detect the SiR-DNA labeled hiPSCs from all imaged wells in each well (1), filter the images by applying a Sliding Parabola algorithm (2) or a Mean Smoothing algorithm (3) and then identify the area occupied by the whole Image Region (4). qPCR quantification of expression markers of ectodermal SOX1 (C) and PAX6 (D), endodermal SOX17 (E) and LEFTY1 (F) and mesodermal NCAM (G), TBXT (H) and CXCR4 (I) markers from BJ WT hiPSCs cultured in STEMdiffTM Trilineage Differentiation Kit (STEMCELL). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: .. Scale bar, 1 mm. (B) Exemplification of the image processing steps done by the Harmony v4.0 software script to detect the SiR-DNA labeled hiPSCs from all imaged wells in each well (1), filter the images by applying a Sliding Parabola algorithm (2) or a Mean Smoothing algorithm (3) and then identify the area occupied by the whole Image Region (4). qPCR quantification of expression markers of ectodermal SOX1 (C) and PAX6 (D), endodermal SOX17 (E) and LEFTY1 (F) and mesodermal NCAM (G), TBXT (H) and CXCR4 (I) markers from BJ WT hiPSCs cultured in STEMdiffTM Trilineage Differentiation Kit (STEMCELL). ..

    Expressing:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: .. Scale bar, 1 mm. (B) Exemplification of the image processing steps done by the Harmony v4.0 software script to detect the SiR-DNA labeled hiPSCs from all imaged wells in each well (1), filter the images by applying a Sliding Parabola algorithm (2) or a Mean Smoothing algorithm (3) and then identify the area occupied by the whole Image Region (4). qPCR quantification of expression markers of ectodermal SOX1 (C) and PAX6 (D), endodermal SOX17 (E) and LEFTY1 (F) and mesodermal NCAM (G), TBXT (H) and CXCR4 (I) markers from BJ WT hiPSCs cultured in STEMdiffTM Trilineage Differentiation Kit (STEMCELL). ..

    Cell Culture:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: .. Scale bar, 1 mm. (B) Exemplification of the image processing steps done by the Harmony v4.0 software script to detect the SiR-DNA labeled hiPSCs from all imaged wells in each well (1), filter the images by applying a Sliding Parabola algorithm (2) or a Mean Smoothing algorithm (3) and then identify the area occupied by the whole Image Region (4). qPCR quantification of expression markers of ectodermal SOX1 (C) and PAX6 (D), endodermal SOX17 (E) and LEFTY1 (F) and mesodermal NCAM (G), TBXT (H) and CXCR4 (I) markers from BJ WT hiPSCs cultured in STEMdiffTM Trilineage Differentiation Kit (STEMCELL). ..

    Cloning:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-IgG Santa Cruz sc2025; RRID: AB_737182 Mouse anti-SMN Novus Biologicals NB100-1936; RRID: AB_531374 Rabbit anti-Gemin2 Abcam ab150383; RRID: AB_3099716 Rat anti-SOX2 Invitrogen 14-9811-82; RRID: AB_11219471 Mouse anti-NESTIN Millipore MAB353; RRID: AB_94911 Rabbit anti-ISL1 Abcam ab109517; RRID: AB_10866454 Mouse anti-NKX6.1 Developmental Studies Hybridoma Bank F55A10; RRID: AB_532378 Mouse anti-MAP2 Novus Biologicals NBP2-25156; RRID: AB_3099715 Rabbit anti-TBXT Cell Signaling 81694; RRID: AB_2799983 Mouse anti-ACTA2 Sigma-Aldrich A2547; RRID: AB_476701 Rabbit anti-CHAT Proteintech 20747-1-AP; RRID: AB_10898169 Mouse anti-SMI32/anti-Neurofilament H (NF-H) Biolegend 801701; RRID: AB_2564642 Rabbit anti-Desmin Abcam ab15200; RRID: AB_301744 Mouse anti-Titin Novus Biologicals NB600-1206; RRID: AB_10003288 Mouse anti-b-Actin Cell Signaling 8H10D10; RRID: AB_2242334 Rabbit anti-b-Tubulin Cell Signaling 2146; RRID: AB_2210545 Chemicals, peptides, and recombinant proteins mTESR1 STEMCELL Technologies 85850 ReLeSR STEMCELL Technologies 100–0484 Advanced DMEM/F12 Thermo Fisher Scientific 12634028 Neurobasal Medium Thermo Fisher Scientific 21103049 GlutaMAX Thermo Fisher Scientific 35050087 2-Mercaptoehtanol Life Technologies 21985023 GlutaMAX Thermo Fisher Scientific 35050087 Ascorbic Acid SigmaAldrich A4403 B27 Thermo Fisher Scientific 17504044 N2 Thermo Fisher Scientific 17502048 Penicillin-Streptomycin Life Technologies 15140122 Matrigel Corning 354234 TRIzol Life Technologies 15596026 Laminin Thermo Fisher Scientific 23017015 Poly-D-lysine Sigma-Aldrich A-003-E TissueTek OCT Sakura Finetek 4583 Sucrose Millipore 107651 MLN-4924 Active Biochem MLN-4924 Cycloheximide Sigma-Aldrich C1988 Y-27632 (ROCKi) Hölzel A11001-50 FGF-2 Millipore 32160702 SB431542 Bio-Techne 1614/10 LDN193189 Hölzel M1873) RA Sigma-Aldrich R2625 SAG Millipore 566660 Ara-C Sigma-Aldrich C1768 (Continued on next page) Cell Reports Medicine 5, 101659, August 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAPT Tocris 2634/10 BDNF Qkine QK050 GDNF Qkine QK051 Papain Worthington LK003178 DNase Worthington LK003172 Accutase Corning 25-058-Cl Vectashield Antifade Mounting Medium Biozol Diagnostica VEC-H-1000 SiR-DNA Spyrochrome SC007 Hoechst33342 Life Technologies H3570 Critical commercial assays STEMdiffTM Trilineage Differentiation Kit STEMCELL Technologies 05230 Deposited data scRNA-seq This paper EGAS00001007259 Experimental models: Cell lines BJ siPS-D Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi005-A, Table S2 1016A Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi007-A, Table S2 38D-I Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi015-A, Table S2 51N-II Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi017-A, Table S2 39C-III Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi016-A, Table S2 CRTD1 CRTD Stem Cell Core Facility (Volkner et al.81) CRTDi004-A, Table S2 BJ-SMN:Clover This paper N/A 38D-I isogenic corrected clone #2 This paper N/A 38D-I Isogenic corrected clone #4 This paper N/A 51N-II Isogenic corrected clone #1 This paper N/A 51N-II Isogenic corrected clone 2 This paper N/A 39C-III Isogenic corrected clone 9 This paper N/A 39C-III Isogenic corrected clone 10 This paper N/A Experimental models: Organisms/strains Mouse: Smn+/ ;hSMN2+/+; hSMND7+/+; Hb9:GFP+ Rodriguez-Muela et al.18,37 N/A Oligonucleotides Single-stranded DNA oligos for cloning IDT See Table S3 Primers for PCR and Sanger Sequencing IDT See Table S3 Primer for RT-qPCR IDT See Table S3 Recombinant DNA pTG-Cr-SMN This paper N/A pTG-HR-SMN:Clover This paper N/A Software and algorithms GraphPad Prism 10.0.3 Graphpad Software, Inc. https://www.graphpad.com/features Geneious Prime 2023.0.3 Biomatters Ltd https://www.geneious.com/ ImageJ software NIH https://imagej.net/ij/ Arivis Vision4D 3.3.0 Zeiss https://www.arivis.com/ Arivis SIS Zeiss https://www.arivis.com/products/ sis-converter (Continued on next page) e2 Cell Reports Medicine 5, 101659, August 20, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Columbus Analysis System PerkinElmer https://www.perkinelmer.com/de/lab- products-and-services/product-support. html#Columbus CellRanger software (v7.1.0) 10X Genomics https://www.10xgenomics.com/support Seurat pipeline (v. 4.3.0) Satija Lab https://satijalab.org/seurat/ R (v. 4.2.2, with Bioconductor 3.16) The R Foundation https://www.r-project.org ZEN Zeiss https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html Article ll OPEN ACCESS

    Polymerase Chain Reaction:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-IgG Santa Cruz sc2025; RRID: AB_737182 Mouse anti-SMN Novus Biologicals NB100-1936; RRID: AB_531374 Rabbit anti-Gemin2 Abcam ab150383; RRID: AB_3099716 Rat anti-SOX2 Invitrogen 14-9811-82; RRID: AB_11219471 Mouse anti-NESTIN Millipore MAB353; RRID: AB_94911 Rabbit anti-ISL1 Abcam ab109517; RRID: AB_10866454 Mouse anti-NKX6.1 Developmental Studies Hybridoma Bank F55A10; RRID: AB_532378 Mouse anti-MAP2 Novus Biologicals NBP2-25156; RRID: AB_3099715 Rabbit anti-TBXT Cell Signaling 81694; RRID: AB_2799983 Mouse anti-ACTA2 Sigma-Aldrich A2547; RRID: AB_476701 Rabbit anti-CHAT Proteintech 20747-1-AP; RRID: AB_10898169 Mouse anti-SMI32/anti-Neurofilament H (NF-H) Biolegend 801701; RRID: AB_2564642 Rabbit anti-Desmin Abcam ab15200; RRID: AB_301744 Mouse anti-Titin Novus Biologicals NB600-1206; RRID: AB_10003288 Mouse anti-b-Actin Cell Signaling 8H10D10; RRID: AB_2242334 Rabbit anti-b-Tubulin Cell Signaling 2146; RRID: AB_2210545 Chemicals, peptides, and recombinant proteins mTESR1 STEMCELL Technologies 85850 ReLeSR STEMCELL Technologies 100–0484 Advanced DMEM/F12 Thermo Fisher Scientific 12634028 Neurobasal Medium Thermo Fisher Scientific 21103049 GlutaMAX Thermo Fisher Scientific 35050087 2-Mercaptoehtanol Life Technologies 21985023 GlutaMAX Thermo Fisher Scientific 35050087 Ascorbic Acid SigmaAldrich A4403 B27 Thermo Fisher Scientific 17504044 N2 Thermo Fisher Scientific 17502048 Penicillin-Streptomycin Life Technologies 15140122 Matrigel Corning 354234 TRIzol Life Technologies 15596026 Laminin Thermo Fisher Scientific 23017015 Poly-D-lysine Sigma-Aldrich A-003-E TissueTek OCT Sakura Finetek 4583 Sucrose Millipore 107651 MLN-4924 Active Biochem MLN-4924 Cycloheximide Sigma-Aldrich C1988 Y-27632 (ROCKi) Hölzel A11001-50 FGF-2 Millipore 32160702 SB431542 Bio-Techne 1614/10 LDN193189 Hölzel M1873) RA Sigma-Aldrich R2625 SAG Millipore 566660 Ara-C Sigma-Aldrich C1768 (Continued on next page) Cell Reports Medicine 5, 101659, August 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAPT Tocris 2634/10 BDNF Qkine QK050 GDNF Qkine QK051 Papain Worthington LK003178 DNase Worthington LK003172 Accutase Corning 25-058-Cl Vectashield Antifade Mounting Medium Biozol Diagnostica VEC-H-1000 SiR-DNA Spyrochrome SC007 Hoechst33342 Life Technologies H3570 Critical commercial assays STEMdiffTM Trilineage Differentiation Kit STEMCELL Technologies 05230 Deposited data scRNA-seq This paper EGAS00001007259 Experimental models: Cell lines BJ siPS-D Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi005-A, Table S2 1016A Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi007-A, Table S2 38D-I Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi015-A, Table S2 51N-II Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi017-A, Table S2 39C-III Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi016-A, Table S2 CRTD1 CRTD Stem Cell Core Facility (Volkner et al.81) CRTDi004-A, Table S2 BJ-SMN:Clover This paper N/A 38D-I isogenic corrected clone #2 This paper N/A 38D-I Isogenic corrected clone #4 This paper N/A 51N-II Isogenic corrected clone #1 This paper N/A 51N-II Isogenic corrected clone 2 This paper N/A 39C-III Isogenic corrected clone 9 This paper N/A 39C-III Isogenic corrected clone 10 This paper N/A Experimental models: Organisms/strains Mouse: Smn+/ ;hSMN2+/+; hSMND7+/+; Hb9:GFP+ Rodriguez-Muela et al.18,37 N/A Oligonucleotides Single-stranded DNA oligos for cloning IDT See Table S3 Primers for PCR and Sanger Sequencing IDT See Table S3 Primer for RT-qPCR IDT See Table S3 Recombinant DNA pTG-Cr-SMN This paper N/A pTG-HR-SMN:Clover This paper N/A Software and algorithms GraphPad Prism 10.0.3 Graphpad Software, Inc. https://www.graphpad.com/features Geneious Prime 2023.0.3 Biomatters Ltd https://www.geneious.com/ ImageJ software NIH https://imagej.net/ij/ Arivis Vision4D 3.3.0 Zeiss https://www.arivis.com/ Arivis SIS Zeiss https://www.arivis.com/products/ sis-converter (Continued on next page) e2 Cell Reports Medicine 5, 101659, August 20, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Columbus Analysis System PerkinElmer https://www.perkinelmer.com/de/lab- products-and-services/product-support. html#Columbus CellRanger software (v7.1.0) 10X Genomics https://www.10xgenomics.com/support Seurat pipeline (v. 4.3.0) Satija Lab https://satijalab.org/seurat/ R (v. 4.2.2, with Bioconductor 3.16) The R Foundation https://www.r-project.org ZEN Zeiss https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html Article ll OPEN ACCESS

    Sequencing:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-IgG Santa Cruz sc2025; RRID: AB_737182 Mouse anti-SMN Novus Biologicals NB100-1936; RRID: AB_531374 Rabbit anti-Gemin2 Abcam ab150383; RRID: AB_3099716 Rat anti-SOX2 Invitrogen 14-9811-82; RRID: AB_11219471 Mouse anti-NESTIN Millipore MAB353; RRID: AB_94911 Rabbit anti-ISL1 Abcam ab109517; RRID: AB_10866454 Mouse anti-NKX6.1 Developmental Studies Hybridoma Bank F55A10; RRID: AB_532378 Mouse anti-MAP2 Novus Biologicals NBP2-25156; RRID: AB_3099715 Rabbit anti-TBXT Cell Signaling 81694; RRID: AB_2799983 Mouse anti-ACTA2 Sigma-Aldrich A2547; RRID: AB_476701 Rabbit anti-CHAT Proteintech 20747-1-AP; RRID: AB_10898169 Mouse anti-SMI32/anti-Neurofilament H (NF-H) Biolegend 801701; RRID: AB_2564642 Rabbit anti-Desmin Abcam ab15200; RRID: AB_301744 Mouse anti-Titin Novus Biologicals NB600-1206; RRID: AB_10003288 Mouse anti-b-Actin Cell Signaling 8H10D10; RRID: AB_2242334 Rabbit anti-b-Tubulin Cell Signaling 2146; RRID: AB_2210545 Chemicals, peptides, and recombinant proteins mTESR1 STEMCELL Technologies 85850 ReLeSR STEMCELL Technologies 100–0484 Advanced DMEM/F12 Thermo Fisher Scientific 12634028 Neurobasal Medium Thermo Fisher Scientific 21103049 GlutaMAX Thermo Fisher Scientific 35050087 2-Mercaptoehtanol Life Technologies 21985023 GlutaMAX Thermo Fisher Scientific 35050087 Ascorbic Acid SigmaAldrich A4403 B27 Thermo Fisher Scientific 17504044 N2 Thermo Fisher Scientific 17502048 Penicillin-Streptomycin Life Technologies 15140122 Matrigel Corning 354234 TRIzol Life Technologies 15596026 Laminin Thermo Fisher Scientific 23017015 Poly-D-lysine Sigma-Aldrich A-003-E TissueTek OCT Sakura Finetek 4583 Sucrose Millipore 107651 MLN-4924 Active Biochem MLN-4924 Cycloheximide Sigma-Aldrich C1988 Y-27632 (ROCKi) Hölzel A11001-50 FGF-2 Millipore 32160702 SB431542 Bio-Techne 1614/10 LDN193189 Hölzel M1873) RA Sigma-Aldrich R2625 SAG Millipore 566660 Ara-C Sigma-Aldrich C1768 (Continued on next page) Cell Reports Medicine 5, 101659, August 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAPT Tocris 2634/10 BDNF Qkine QK050 GDNF Qkine QK051 Papain Worthington LK003178 DNase Worthington LK003172 Accutase Corning 25-058-Cl Vectashield Antifade Mounting Medium Biozol Diagnostica VEC-H-1000 SiR-DNA Spyrochrome SC007 Hoechst33342 Life Technologies H3570 Critical commercial assays STEMdiffTM Trilineage Differentiation Kit STEMCELL Technologies 05230 Deposited data scRNA-seq This paper EGAS00001007259 Experimental models: Cell lines BJ siPS-D Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi005-A, Table S2 1016A Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi007-A, Table S2 38D-I Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi015-A, Table S2 51N-II Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi017-A, Table S2 39C-III Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi016-A, Table S2 CRTD1 CRTD Stem Cell Core Facility (Volkner et al.81) CRTDi004-A, Table S2 BJ-SMN:Clover This paper N/A 38D-I isogenic corrected clone #2 This paper N/A 38D-I Isogenic corrected clone #4 This paper N/A 51N-II Isogenic corrected clone #1 This paper N/A 51N-II Isogenic corrected clone 2 This paper N/A 39C-III Isogenic corrected clone 9 This paper N/A 39C-III Isogenic corrected clone 10 This paper N/A Experimental models: Organisms/strains Mouse: Smn+/ ;hSMN2+/+; hSMND7+/+; Hb9:GFP+ Rodriguez-Muela et al.18,37 N/A Oligonucleotides Single-stranded DNA oligos for cloning IDT See Table S3 Primers for PCR and Sanger Sequencing IDT See Table S3 Primer for RT-qPCR IDT See Table S3 Recombinant DNA pTG-Cr-SMN This paper N/A pTG-HR-SMN:Clover This paper N/A Software and algorithms GraphPad Prism 10.0.3 Graphpad Software, Inc. https://www.graphpad.com/features Geneious Prime 2023.0.3 Biomatters Ltd https://www.geneious.com/ ImageJ software NIH https://imagej.net/ij/ Arivis Vision4D 3.3.0 Zeiss https://www.arivis.com/ Arivis SIS Zeiss https://www.arivis.com/products/ sis-converter (Continued on next page) e2 Cell Reports Medicine 5, 101659, August 20, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Columbus Analysis System PerkinElmer https://www.perkinelmer.com/de/lab- products-and-services/product-support. html#Columbus CellRanger software (v7.1.0) 10X Genomics https://www.10xgenomics.com/support Seurat pipeline (v. 4.3.0) Satija Lab https://satijalab.org/seurat/ R (v. 4.2.2, with Bioconductor 3.16) The R Foundation https://www.r-project.org ZEN Zeiss https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html Article ll OPEN ACCESS

    Quantitative RT-PCR:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-IgG Santa Cruz sc2025; RRID: AB_737182 Mouse anti-SMN Novus Biologicals NB100-1936; RRID: AB_531374 Rabbit anti-Gemin2 Abcam ab150383; RRID: AB_3099716 Rat anti-SOX2 Invitrogen 14-9811-82; RRID: AB_11219471 Mouse anti-NESTIN Millipore MAB353; RRID: AB_94911 Rabbit anti-ISL1 Abcam ab109517; RRID: AB_10866454 Mouse anti-NKX6.1 Developmental Studies Hybridoma Bank F55A10; RRID: AB_532378 Mouse anti-MAP2 Novus Biologicals NBP2-25156; RRID: AB_3099715 Rabbit anti-TBXT Cell Signaling 81694; RRID: AB_2799983 Mouse anti-ACTA2 Sigma-Aldrich A2547; RRID: AB_476701 Rabbit anti-CHAT Proteintech 20747-1-AP; RRID: AB_10898169 Mouse anti-SMI32/anti-Neurofilament H (NF-H) Biolegend 801701; RRID: AB_2564642 Rabbit anti-Desmin Abcam ab15200; RRID: AB_301744 Mouse anti-Titin Novus Biologicals NB600-1206; RRID: AB_10003288 Mouse anti-b-Actin Cell Signaling 8H10D10; RRID: AB_2242334 Rabbit anti-b-Tubulin Cell Signaling 2146; RRID: AB_2210545 Chemicals, peptides, and recombinant proteins mTESR1 STEMCELL Technologies 85850 ReLeSR STEMCELL Technologies 100–0484 Advanced DMEM/F12 Thermo Fisher Scientific 12634028 Neurobasal Medium Thermo Fisher Scientific 21103049 GlutaMAX Thermo Fisher Scientific 35050087 2-Mercaptoehtanol Life Technologies 21985023 GlutaMAX Thermo Fisher Scientific 35050087 Ascorbic Acid SigmaAldrich A4403 B27 Thermo Fisher Scientific 17504044 N2 Thermo Fisher Scientific 17502048 Penicillin-Streptomycin Life Technologies 15140122 Matrigel Corning 354234 TRIzol Life Technologies 15596026 Laminin Thermo Fisher Scientific 23017015 Poly-D-lysine Sigma-Aldrich A-003-E TissueTek OCT Sakura Finetek 4583 Sucrose Millipore 107651 MLN-4924 Active Biochem MLN-4924 Cycloheximide Sigma-Aldrich C1988 Y-27632 (ROCKi) Hölzel A11001-50 FGF-2 Millipore 32160702 SB431542 Bio-Techne 1614/10 LDN193189 Hölzel M1873) RA Sigma-Aldrich R2625 SAG Millipore 566660 Ara-C Sigma-Aldrich C1768 (Continued on next page) Cell Reports Medicine 5, 101659, August 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAPT Tocris 2634/10 BDNF Qkine QK050 GDNF Qkine QK051 Papain Worthington LK003178 DNase Worthington LK003172 Accutase Corning 25-058-Cl Vectashield Antifade Mounting Medium Biozol Diagnostica VEC-H-1000 SiR-DNA Spyrochrome SC007 Hoechst33342 Life Technologies H3570 Critical commercial assays STEMdiffTM Trilineage Differentiation Kit STEMCELL Technologies 05230 Deposited data scRNA-seq This paper EGAS00001007259 Experimental models: Cell lines BJ siPS-D Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi005-A, Table S2 1016A Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi007-A, Table S2 38D-I Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi015-A, Table S2 51N-II Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi017-A, Table S2 39C-III Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi016-A, Table S2 CRTD1 CRTD Stem Cell Core Facility (Volkner et al.81) CRTDi004-A, Table S2 BJ-SMN:Clover This paper N/A 38D-I isogenic corrected clone #2 This paper N/A 38D-I Isogenic corrected clone #4 This paper N/A 51N-II Isogenic corrected clone #1 This paper N/A 51N-II Isogenic corrected clone 2 This paper N/A 39C-III Isogenic corrected clone 9 This paper N/A 39C-III Isogenic corrected clone 10 This paper N/A Experimental models: Organisms/strains Mouse: Smn+/ ;hSMN2+/+; hSMND7+/+; Hb9:GFP+ Rodriguez-Muela et al.18,37 N/A Oligonucleotides Single-stranded DNA oligos for cloning IDT See Table S3 Primers for PCR and Sanger Sequencing IDT See Table S3 Primer for RT-qPCR IDT See Table S3 Recombinant DNA pTG-Cr-SMN This paper N/A pTG-HR-SMN:Clover This paper N/A Software and algorithms GraphPad Prism 10.0.3 Graphpad Software, Inc. https://www.graphpad.com/features Geneious Prime 2023.0.3 Biomatters Ltd https://www.geneious.com/ ImageJ software NIH https://imagej.net/ij/ Arivis Vision4D 3.3.0 Zeiss https://www.arivis.com/ Arivis SIS Zeiss https://www.arivis.com/products/ sis-converter (Continued on next page) e2 Cell Reports Medicine 5, 101659, August 20, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Columbus Analysis System PerkinElmer https://www.perkinelmer.com/de/lab- products-and-services/product-support. html#Columbus CellRanger software (v7.1.0) 10X Genomics https://www.10xgenomics.com/support Seurat pipeline (v. 4.3.0) Satija Lab https://satijalab.org/seurat/ R (v. 4.2.2, with Bioconductor 3.16) The R Foundation https://www.r-project.org ZEN Zeiss https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html Article ll OPEN ACCESS

    Recombinant:

    Article Title: Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-IgG Santa Cruz sc2025; RRID: AB_737182 Mouse anti-SMN Novus Biologicals NB100-1936; RRID: AB_531374 Rabbit anti-Gemin2 Abcam ab150383; RRID: AB_3099716 Rat anti-SOX2 Invitrogen 14-9811-82; RRID: AB_11219471 Mouse anti-NESTIN Millipore MAB353; RRID: AB_94911 Rabbit anti-ISL1 Abcam ab109517; RRID: AB_10866454 Mouse anti-NKX6.1 Developmental Studies Hybridoma Bank F55A10; RRID: AB_532378 Mouse anti-MAP2 Novus Biologicals NBP2-25156; RRID: AB_3099715 Rabbit anti-TBXT Cell Signaling 81694; RRID: AB_2799983 Mouse anti-ACTA2 Sigma-Aldrich A2547; RRID: AB_476701 Rabbit anti-CHAT Proteintech 20747-1-AP; RRID: AB_10898169 Mouse anti-SMI32/anti-Neurofilament H (NF-H) Biolegend 801701; RRID: AB_2564642 Rabbit anti-Desmin Abcam ab15200; RRID: AB_301744 Mouse anti-Titin Novus Biologicals NB600-1206; RRID: AB_10003288 Mouse anti-b-Actin Cell Signaling 8H10D10; RRID: AB_2242334 Rabbit anti-b-Tubulin Cell Signaling 2146; RRID: AB_2210545 Chemicals, peptides, and recombinant proteins mTESR1 STEMCELL Technologies 85850 ReLeSR STEMCELL Technologies 100–0484 Advanced DMEM/F12 Thermo Fisher Scientific 12634028 Neurobasal Medium Thermo Fisher Scientific 21103049 GlutaMAX Thermo Fisher Scientific 35050087 2-Mercaptoehtanol Life Technologies 21985023 GlutaMAX Thermo Fisher Scientific 35050087 Ascorbic Acid SigmaAldrich A4403 B27 Thermo Fisher Scientific 17504044 N2 Thermo Fisher Scientific 17502048 Penicillin-Streptomycin Life Technologies 15140122 Matrigel Corning 354234 TRIzol Life Technologies 15596026 Laminin Thermo Fisher Scientific 23017015 Poly-D-lysine Sigma-Aldrich A-003-E TissueTek OCT Sakura Finetek 4583 Sucrose Millipore 107651 MLN-4924 Active Biochem MLN-4924 Cycloheximide Sigma-Aldrich C1988 Y-27632 (ROCKi) Hölzel A11001-50 FGF-2 Millipore 32160702 SB431542 Bio-Techne 1614/10 LDN193189 Hölzel M1873) RA Sigma-Aldrich R2625 SAG Millipore 566660 Ara-C Sigma-Aldrich C1768 (Continued on next page) Cell Reports Medicine 5, 101659, August 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAPT Tocris 2634/10 BDNF Qkine QK050 GDNF Qkine QK051 Papain Worthington LK003178 DNase Worthington LK003172 Accutase Corning 25-058-Cl Vectashield Antifade Mounting Medium Biozol Diagnostica VEC-H-1000 SiR-DNA Spyrochrome SC007 Hoechst33342 Life Technologies H3570 Critical commercial assays STEMdiffTM Trilineage Differentiation Kit STEMCELL Technologies 05230 Deposited data scRNA-seq This paper EGAS00001007259 Experimental models: Cell lines BJ siPS-D Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi005-A, Table S2 1016A Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi007-A, Table S2 38D-I Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi015-A, Table S2 51N-II Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi017-A, Table S2 39C-III Laboratory of Lee L. Rubin (Rodriguez-Muela et al.18,37) HVRDi016-A, Table S2 CRTD1 CRTD Stem Cell Core Facility (Volkner et al.81) CRTDi004-A, Table S2 BJ-SMN:Clover This paper N/A 38D-I isogenic corrected clone #2 This paper N/A 38D-I Isogenic corrected clone #4 This paper N/A 51N-II Isogenic corrected clone #1 This paper N/A 51N-II Isogenic corrected clone 2 This paper N/A 39C-III Isogenic corrected clone 9 This paper N/A 39C-III Isogenic corrected clone 10 This paper N/A Experimental models: Organisms/strains Mouse: Smn+/ ;hSMN2+/+; hSMND7+/+; Hb9:GFP+ Rodriguez-Muela et al.18,37 N/A Oligonucleotides Single-stranded DNA oligos for cloning IDT See Table S3 Primers for PCR and Sanger Sequencing IDT See Table S3 Primer for RT-qPCR IDT See Table S3 Recombinant DNA pTG-Cr-SMN This paper N/A pTG-HR-SMN:Clover This paper N/A Software and algorithms GraphPad Prism 10.0.3 Graphpad Software, Inc. https://www.graphpad.com/features Geneious Prime 2023.0.3 Biomatters Ltd https://www.geneious.com/ ImageJ software NIH https://imagej.net/ij/ Arivis Vision4D 3.3.0 Zeiss https://www.arivis.com/ Arivis SIS Zeiss https://www.arivis.com/products/ sis-converter (Continued on next page) e2 Cell Reports Medicine 5, 101659, August 20, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Columbus Analysis System PerkinElmer https://www.perkinelmer.com/de/lab- products-and-services/product-support. html#Columbus CellRanger software (v7.1.0) 10X Genomics https://www.10xgenomics.com/support Seurat pipeline (v. 4.3.0) Satija Lab https://satijalab.org/seurat/ R (v. 4.2.2, with Bioconductor 3.16) The R Foundation https://www.r-project.org ZEN Zeiss https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html Article ll OPEN ACCESS

    Generated:

    Article Title: Generation of human induced pluripotent stem cell (hiPSC) lines (UKMi009-A and UKMi011-A) harboring a homozygous and heterozygous HCN4 variant from a family with inherited sinus node dysfunction (SND).
    Article Snippet: Immunofluorescence images were acquired by confocal laser scanning microscopy (Nikon Ti2 Eclipse) Tables 1 and 2. .. Generated hiPSCs were functionally characterized by directed differentiation into all three germ layers using STEMdiffTM Trilineage Differentiation Kit (Stemcell Technologies). ..



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